Today I was alerted of a citation of my paper on Bulimulus phylogeny, and when I looked up the citation I found a paper by Martins et al. which will appear in the Journal of Invertebrate Pathology.
“The terrestrial gastropod Bulimulus tenuissimus is widespread in South America. It is an intermediate host of many parasites, but there are no records of infection of this snail by Angiostrongylus cantonensis, despite the occurrence of this parasite and angiostrongyliasis cases in the same areas in which B. tenuissimus occurs. For this reason, it is important investigate the susceptibility of B. tenuissimus to A. cantonensis-infection, since it can be used as intermediate host of A. cantonensis, increasing the list of terrestrial gastropods that infect wild and domestic animals and humans with this parasite. The purpose of this study was to evaluate the susceptibility of B. tenuissimus to experimental infection with L1 larvae of A. cantonensis. The snails were exposed to 1,200 L1 larvae and it was possible observe many developing larvae in the cephalopedal mass and mantle tissues, with intense hemocyte infiltration and collagen deposition, but no typical granuloma structures were formed. The glucose content and lactate dehydrogenase activity in the hemolymph varied, indicating an increase of anaerobic energy metabolism in the middle of infection, but with a tendency to return to normal values at the end of pre-patent period. This was corroborated by the marked reduction in the glycogen content in the cephalopedal mass and digestive gland in the first and second week after exposure, followed by a slight increase in the third week. The content of pyruvic acid in the hemolymph was 14.84% lower at the end of pre-patent period, and oxalic acid content was 41.14% higher. These results indicate an aerobic to anaerobic transition process. The PAS reaction showed a large amount of glycogen inside the developing larvae and muscular tissues of the cephalopedal mass, indicating that despite the high consumption of this polysaccharide by the parasite, the snail is able to maintain its energy metabolism based on carbohydrates. The results reveal that B. tenuissimus is a robust host, which can live with the developing larvae of A. cantonensisand overcome the metabolic damages resulting from parasitism.”
No phylogenetic related work thus, it seems that the citation was only used to support the wide-spread occurrence of Bulimulus species in different areas and the fact that some of these are easily imported and may act as alien species. I also found a (unjustified) citation in Martins et al. of a paper by Parent & Crispi dealing with the radiation of Galápagos Naesiotus species, which were misidentified as Bulimulus.
Although this seems a case of serendipity, it is interesting to know that Bulimulus tenuissimus is a potential host for Angiostrongylus cantonensis, given the fact that this is a potential health threat for humans. An update will be given once this paper is formally published.
Martins, F.G. et al., 2018. Bulimulus tenuissimus (Mollusca) as a new potential host of Angiostrogylus cantonensis (Nematoda), a histological and metabolic study. – Journal of Invertebrate Pathology